SPAG1 antibody (N-Term)

Catalog No. ABIN651886

This Rabbit Polyclonal antibody specifically detects SPAG1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SPAG1 antibody (N-Term) (ABIN651886)

Target: SPAG1 (Sperm Associated Antigen 1 (SPAG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB24228

Product Details anti-SPAG1 Antibody

Binding Specificity: AA 124-152, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SPAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-152 amino acids from the N-terminal region of human SPAG1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for SPAG1

Target: SPAG1 (Sperm Associated Antigen 1 (SPAG1))

Alternative Name: SPAG1

Background: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq].

Molecular Weight: 103639

Gene ID: 6674

NCBI Accession: NP_003105, NP_757367

UniProt: Q07617