BMI1 antibody

Catalog No. ABIN652702

This Rabbit Polyclonal antibody specifically detects BMI1 in WB, IF, IHC (p) and FACS. It exhibits reactivity toward Human and Mouse and has been mentioned in 3+ publications.

Quick Overview for BMI1 antibody (ABIN652702)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB22858

Product Details anti-BMI1 Antibody

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This BMI1 antibody is generated from rabbits immunized with BMI1 recombinant protein.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:2000. WB: 1:8000. IHC-P: 1:10~50. FC: 1:25. FC: 1:25. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-BMI1 antibody (ABIN652702)

Zhou, Dai, Wu, Ji, Karaplis, Goltzman, Yang, Miao: "Overexpression of Bmi1 in Lymphocytes Stimulates Skeletogenesis by Improving the Osteogenic Microenvironment." in: Scientific reports, Vol. 6, pp. 29171, (2018) (PubMed).

León-González, Jara-Palacios, Abbas, Heredia, Schini-Kerth: "Role of epigenetic regulation on the induction of apoptosis in Jurkat leukemia cells by white grape pomace rich in phenolic compounds." in: Food & function, Vol. 8, Issue 11, pp. 4062-4069, (2018) (PubMed).

Dauleh, Santeramo, Fielding, Ward, Herrmann, Murray, Wilm: "Characterisation of Cultured Mesothelial Cells Derived from the Murine Adult Omentum." in: PLoS ONE, Vol. 11, Issue 7, pp. e0158997, (2017) (PubMed).

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Molecular Weight: 36949

Gene ID: 648

NCBI Accession: NP_001190991, NP_005171

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy