PDHA1 antibody (AA 226-255)

Catalog No. ABIN653604

The Rabbit Polyclonal anti-PDHA1 antibody (Clone RB24303) (ABIN653604) specifically detects PDHA1 in WB. The antibody is reactive with Human samples.

Quick Overview for PDHA1 antibody (AA 226-255) (ABIN653604)

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHA1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB24303

Product Details anti-PDHA1 Antibody

Binding Specificity: AA 226-255

Predicted Reactivity: Zf, B, Pr, M, Pig, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PDHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-255 amino acids from the Central region of human PDHA1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PDHA1

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Alternative Name: PDHA1

Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.

Molecular Weight: 43296

Gene ID: 5160

NCBI Accession: NP_000275, NP_001166925, NP_001166926, NP_001166927

UniProt: P08559

Pathways: Warburg Effect