Angiopoietin 2 antibody (C-Term)

Catalog No. ABIN654062

The Rabbit Polyclonal anti-Angiopoietin 2 antibody has been validated for WB, IHC (p) and FACS. It is suitable to detect Angiopoietin 2 in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for Angiopoietin 2 antibody (C-Term) (ABIN654062)

Target: Angiopoietin 2 (ANGPT2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB17789

Product Details anti-Angiopoietin 2 Antibody

Binding Specificity: AA 404-432, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-432 amino acids from the C-terminal region of human ANGPT2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. IHC-P: 1:50~100. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Angiopoietin 2 antibody (ABIN654062)

Alamo, Gallardo, Di Nicolantonio, Pavón, Casanova, Trias, Mangues, Lopez-Pousa, Villaverde, Vázquez, Bardelli, Céspedes, Mangues: "Higher metastatic efficiency of KRas G12V than KRas G13D in a colorectal cancer model." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 2, pp. 464-76, (2015) (PubMed).

Alamo, Gallardo, Pavón, Casanova, Trias, Mangues, Vázquez, Villaverde, Mangues, Céspedes: "Subcutaneous preconditioning increases invasion and metastatic dissemination in mouse colorectal cancer models." in: Disease models & mechanisms, Vol. 7, Issue 3, pp. 387-96, (2014) (PubMed).

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: ANGPT2

Background: The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

Molecular Weight: 56919

Gene ID: 285

NCBI Accession: NP_001112359, NP_001112360, NP_001138

UniProt: O15123

Pathways: RTK Signaling