NSUN2 antibody (AA 423-451)

Catalog No. ABIN654767

The Rabbit Polyclonal anti-NSUN2 antibody is suitable to detect NSUN2 in samples from Human and Mouse. It has been validated for WB.

Quick Overview for NSUN2 antibody (AA 423-451) (ABIN654767)

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB28701

Product Details anti-NSUN2 Antibody

Binding Specificity: AA 423-451

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-451 amino acids from the Central region of human NSUN2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Alternative Name: NSUN2

Background: Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).

Molecular Weight: 86471

Gene ID: 54888

NCBI Accession: NP_001180384, NP_060225

UniProt: Q08J23