DHCR7 antibody (C-Term)

Catalog No. ABIN655399

This Rabbit Polyclonal antibody specifically detects DHCR7 in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for DHCR7 antibody (C-Term) (ABIN655399)

Target: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHCR7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB17942

Product Details anti-DHCR7 Antibody

Binding Specificity: AA 437-463, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DHCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-463 amino acids from the C-terminal region of human DHCR7.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. IF: 1:10~50. WB: 1:2000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for DHCR7

Target: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Alternative Name: DHCR7

Background: This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS), a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.

Molecular Weight: 54489

Gene ID: 1717

NCBI Accession: NP_001157289, NP_001351

UniProt: Q9UBM7