SIRPB1 antibody (PE)

Catalog No. ABIN6559830

This Mouse Monoclonal antibody specifically detects SIRPB1 in FACS. It exhibits reactivity toward Human.

Quick Overview for SIRPB1 antibody (PE) (ABIN6559830)

Target: SIRPB1 (Signal-Regulatory Protein beta 1 (SIRPB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPB1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: B4B6

Product Details anti-SIRPB1 Antibody (PE)

Purpose: Anti-Hu CD172b PE

Specificity: The mouse monoclonal antibody B4B6 recognizes an extracellular epitope of CD172b, an approximately 50 kDa transmembrane glycoprotein expressed on myeloid cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: NIH-3T3 cells transfected with human CD172b

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for SIRPB1

Target: SIRPB1 (Signal-Regulatory Protein beta 1 (SIRPB1))

Alternative Name: CD172b

Background: Signal regulatory protein beta 1,CD172b, the signal-regulatory protein beta (SIRP beta) is a disulfide-linked homodimer expressed on myeloid cells including monocytes and dendritic cells. Similarly to CD172a, it serves as a negative regulator of tyrosine kinase-coupled signaling processes. Unlike CD172a, the CD172b protein does not possess the cytoplasmic domain, but instead its transmembrane domain can interact with another transmembrane protein DAP-12, which contains ITAM sequences in its intracellular domain and links CD172b to the downstream signaling molecules. The result is e.g. regulation of neutrophil transepithelial migration.,SIRPB1

Gene ID: 10326

UniProt: O00241