PDP antibody (AA 308-336)

Catalog No. ABIN656386

This Rabbit Polyclonal antibody specifically detects PDP in WB and IHC (p). It exhibits reactivity toward Human and Ratand has been mentioned in 1 publication.

Quick Overview for PDP antibody (AA 308-336) (ABIN656386)

Target: PDP (Pyruvate Dehydrogenase Phosphatase (PDP))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB31327

Product Details anti-PDP Antibody

Binding Specificity: AA 308-336

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PDP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the Central region of human PDP1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: PDP1 Antibody (Center) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

References for anti-PDP antibody (ABIN656386)

Shan, Kang, Elf, Xie, Gu, Aguiar, Lonning, Hitosugi, Chung, Arellano, Khoury, Shin, Khuri, Boggon, Fan: "Tyr-94 phosphorylation inhibits pyruvate dehydrogenase phosphatase 1 and promotes tumor growth." in: The Journal of biological chemistry, Vol. 289, Issue 31, pp. 21413-22, (2014) (PubMed).

Target Details for PDP

Target: PDP (Pyruvate Dehydrogenase Phosphatase (PDP))

Alternative Name: PDP1

Background: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported, one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 61054

Gene ID: 54704

NCBI Accession: NP_001155251, NP_001155252, NP_001155253, NP_060914

UniProt: Q9P0J1