PDP antibody (Middle Region)

Catalog No. ABIN954075

The Rabbit Polyclonal anti-PDP antibody (ABIN954075) specifically detects PDP in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for PDP antibody (Middle Region) (ABIN954075)

Target: PDP (Pyruvate Dehydrogenase Phosphatase (PDP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PDP Antibody

Binding Specificity: AA 366-395, Middle Region

Specificity: This antibody recognizes Human PDP1 (Center).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 366-395 amino acids from the Central region of Human PDP1

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PDP

Target: PDP (Pyruvate Dehydrogenase Phosphatase (PDP))

Alternative Name: PDP1

Background: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported, one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.Synonyms: Mitochondrial [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, PDP, PDPC1, PPM2C, Protein phosphatase 2C, Pyruvate dehydrogenase phosphatase catalytic subunit 1

Molecular Weight: 61054 Da

Gene ID: 54704

NCBI Accession: NP_001155250