PSME3 antibody

Catalog No. ABIN6566551

This Rabbit Polyclonal antibody specifically detects PSME3 in WB and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for PSME3 antibody (ABIN6566551)

Target: PSME3

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSME3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PSME3 Antibody

Purification: Affinity purification

Immunogen: Recombinant protein of human PSME3

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000 IF 1:20 - 1:50

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for PSME3

Target: PSME3

Alternative Name: PSME3

Background:

Synonyms: 11S regulator complex gamma subunit,11S regulator complex subunit gamma,Activator of multicatalytic protease subunit 3,Ki,Ki antigen,Ki nuclear autoantigen,Ki,PA28 gamma,PA28 gamma,PA28g,PA28gamma,Proteasome (prosome,macropain) activator subunit 3 (PA28 gamma,Ki),Proteasome (prosome,macropain) activator subunit 3,Proteasome activator 28 gamma,Proteasome activator 28 subunit gamma,Proteasome activator complex subunit 3,Proteasome activator subunit 3,PSME3,PSME3,REG GAMMA,REG-gamma

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants.

Molecular Weight:

Observed_MW: 32kDa

Calculated_MW: 29kDa/30kDa

Gene ID: 10197

UniProt: P61289

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Hepatitis C, Synthesis of DNA