KRT12 antibody (C-Term)

Catalog No. ABIN656663

The Rabbit Polyclonal anti-KRT12 antibody has been validated for WB, FACS and IHC (p). It is suitable to detect KRT12 in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for KRT12 antibody (C-Term) (ABIN656663)

Target: KRT12 (Keratin 12 (KRT12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT12 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB32401

Product Details anti-KRT12 Antibody

Binding Specificity: AA 442-471, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This KRT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the C-terminal region of human KRT12.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. WB: 1:2000. WB: 1:500. IHC-P: 1:10~50. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: KRT12 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

References for anti-KRT12 antibody (ABIN656663)

Figueiredo, Bojic, Rooney, Wilshaw, Connon, Gouveia, Paterson, Lepert, Mudhar, Figueiredo, Lako: "Gamma-irradiated human amniotic membrane decellularised with sodium dodecyl sulfate is a more efficient substrate for the ex vivo expansion of limbal stem cells." in: Acta biomaterialia, Vol. 61, pp. 124-133, (2018) (PubMed).

Yu, Bojic, Figueiredo, Rooney, de Havilland, Dickinson, Figueiredo, Lako: "An important role for adenine, cholera toxin, hydrocortisone and triiodothyronine in the proliferation, self-renewal and differentiation of limbal stem cells in vitro." in: Experimental eye research, Vol. 152, pp. 113-122, (2016) (PubMed).

Target Details for KRT12

Target: KRT12 (Keratin 12 (KRT12))

Alternative Name: KRT12

Background: KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

Molecular Weight: 53511

Gene ID: 3859

NCBI Accession: NP_000214

UniProt: Q99456