NeuN antibody

Catalog No. ABIN6566704

This Rabbit Polyclonal antibody specifically detects NeuN in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NeuN antibody (ABIN6566704)

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NeuN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NeuN Antibody

Purification: Affinity purification

Immunogen: A synthetic peptide of human RBFOX3

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000 IF 1:50 - 1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for NeuN

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Alternative Name: RBFOX3

Background:

Synonyms: FLJ56884,FLJ58356,Fox-1 homolog C,fox1 homolog C,Fox3,FOX3NeuN,hexaribonucleotide binding protein 3,HRNBP3,NEUN,neuronal nuclei,Rbfox3,RFOX3,RNA binding protein fox-1 homolog 3,RNA binding protein,fox 1 homolog (C. elegans) 3

Background: This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Molecular Weight:

Observed_MW: 34kDa

Calculated_MW: 33kDa/35kDa

Gene ID: 146713

UniProt: A6NFN3