APOB antibody

Catalog No. ABIN6567867

The Rabbit Polyclonal anti-APOB antibody (ABIN6567867) specifically detects APOB in IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for APOB antibody (ABIN6567867)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOB antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-APOB Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human APOB (NP_000375.2).

Isotype: IgG

Application Details

Application Notes: IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: APOB

Background: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Molecular Weight:

Observed_MW: 110kDa

Calculated_MW: 515kDa

Gene ID: 338

UniProt: P04114

Pathways: Lipid Metabolism