PPARA antibody (AA 153-181)

Catalog No. ABIN656834

The Rabbit Polyclonal anti-PPARA antibody has been validated for WB and FACS. It is suitable to detect PPARA in samples from Mouse. There is 1 publication available.

Quick Overview for PPARA antibody (AA 153-181) (ABIN656834)

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: RB21340

Product Details anti-PPARA Antibody

Binding Specificity: AA 153-181

Predicted Reactivity: Rat, X

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PPARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-181 amino acids from the Central region of human PPARA.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: PPARA Antibody (Center) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

References for anti-PPARA antibody (ABIN656834)

Spruiell, Richardson, Cullen, Awumey, Gonzalez, Gyamfi: "Role of pregnane X receptor in obesity and glucose homeostasis in male mice." in: The Journal of biological chemistry, Vol. 289, Issue 6, pp. 3244-61, (2014) (PubMed).

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPARA

Background: Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers, this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.

Molecular Weight: 52225

Gene ID: 5465

NCBI Accession: NP_001001928, NP_005027

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C