CYBB antibody

Catalog No. ABIN6568819

The Rabbit Polyclonal anti-CYBB antibody (ABIN6568819) specifically detects CYBB in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CYBB antibody (ABIN6568819)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CYBB Antibody

Purification: Affinity purification

Immunogen: A synthetic peptide of human CYBB

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000 IHC 1:50 - 1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: CYBB

Background:

Synonyms: AMCBX2,CGD,CGD91-phox,CY24B,CYBB,Cytochrome b 245,beta polypeptide,Cytochrome b(558) beta chain,Cytochrome b(558) subunit beta,Cytochrome b-245 heavy chain,Cytochrome b558 subunit beta,GP91 PHOX,gp91-1,gp91-phox,GP91PHOX,Heme-binding membrane glycoprotein gp91phox,NADPH oxidase 2,Neutrophil cytochrome b 91 kDa polypeptide,NOX2,p22 phagocyte B-cytochrome,P91 PHOX,p91-PHOX,Superoxide-generating NADPH oxidase heavy chain subunit

Background: Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase, neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.

Molecular Weight:

Observed_MW: 65kDa

Calculated_MW: 65kDa

Gene ID: 1536

UniProt: P04839