RabbitBP4 / RabbitAP48 (AA 1-425) antibody

Catalog No. ABIN189975

The Mouse Monoclonal anti-RabbitBP4 / RabbitAP48 antibody (Clone 15G12) (ABIN189975) specifically detects RabbitBP4 / RabbitAP48 in IF/ICC, IHC (p), IP, WB and EMSA. The antibody is reactive with Human samples.

Quick Overview for RabbitBP4 / RabbitAP48 (AA 1-425) antibody (ABIN189975)

Target: RabbitBP4 / RabbitAP48

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: 15G12

Product Details

Binding Specificity: AA 1-425

Purpose: Anti-RbAp48 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human and mouse RbAp48 and RbAp46.

Cross-Reactivity: Human

Immunogen: GST fusion protein corresponding to aa 1-425 of human RbAp48.

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL. A band of ~48 kDa is detected. Detection of RbAp48 in K562, THP-1, and HL-60 whole cell extracts (30 μg), separated by 10 % SDS-PAGE, with #3111 at 2 μg/mL.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.
Immunohistochemistry: use at 5-10 μg/mL. Detection of RbAp48 in paraffin-embedded human cervical carcinoma with #3111 at 10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: This antibody is stable at -20°C for at least one (1) year, store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Target Details

Target: RabbitBP4 / RabbitAP48

Background: Retinoblastoma-associated protein,Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}., (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.,Nucleus,p105-Rb, p110-RB1, pRb, Rb, pp110

Gene ID: 5925

NCBI Accession: NP_000312

UniProt: P06400