CENPF antibody (AA 1759-2093)

Catalog No. ABIN189989

The Mouse Monoclonal anti-CENPF antibody is suitable to detect CENPF in samples from Human. It has been validated for WB, IP, IHC (p), IHC (fro), IF/ICC and FACS.

Quick Overview for CENPF antibody (AA 1759-2093) (ABIN189989)

Target: CENPF (Centromere Protein F (CENPF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPF antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (fixed cells) (IF/ICC), Flow Cytometry (FACS)

Clone: 14C10

Product Details anti-CENPF Antibody

Binding Specificity: AA 1759-2093

Purpose: Anti-Mitosin Mouse Monoclonal Antibody

Specificity: This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

Cross-Reactivity: Human

Immunogen: GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting: use at 1-10 μg/mL.
Immunoprecipitation: use at 1-10 μg/mL.
Immunohistochemistry: use at 10 μg/mL with frozen or paraffin-embedded sections.
Positive controls: Human tissue containing rapidly proliferating cells, such as lymphoid germinal centers.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -80 °C

Storage Comment: This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENP-F

Background: Centromere protein F,Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. {PubMed:12974617, PubMed:17600710, PubMed:7542657, PubMed:7651420}.,Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.,CENP-F, AH antigen, Kinetochore protein CENPF, Mitosin

Gene ID: 1063

NCBI Accession: NP_057427

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection