ESR2 antibody (AA 1-153)

Catalog No. ABIN189996

The Mouse Monoclonal anti-ESR2 antibody (Clone 6A12) (ABIN189996) specifically detects ESR2 in WB, IHC, IF/ICC and ChIP. The antibody is reactive with Human samples.

Quick Overview for ESR2 antibody (AA 1-153) (ABIN189996)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC), Chromatin Immunoprecipitation (ChIP)

Clone: 6A12

Product Details anti-ESR2 Antibody

Binding Specificity: AA 1-153

Purpose: Anti-Estrogen Receptor beta Mouse Monoclonal Antibody

Specificity: This antibody recognizes human ER-b, a novel receptor (approx. 52 kD) recently isolated from testis. The size and structure of ER-b are very similar to ER-alpha with the ligand and DNA binding domains being highly conserved. The N-terminus of each receptor, which serves as their transactivation domain, are significantly different. Like ER-alpha, ER-b binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements.

Cross-Reactivity: Human

Immunogen: 6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli.

Isotype: IgG2b

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL.
Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -80 °C

Storage Comment: This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: Estrogen receptor beta

Background: Estrogen receptor beta,Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). {PubMed:20074560, PubMed:29261182, PubMed:30113650, PubMed:9325313}., [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. {PubMed:9671811}.,Nucleus,ER-beta, Nuclear receptor subfamily 3 group A member 2

Gene ID: 2100

NCBI Accession: NP_001035365

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling