Histone H4 antibody (H4K12ac)

Catalog No. ABIN6579166

Cited in 7+ publications. The Rabbit Polyclonal anti-Histone H4 antibody (ABIN6579166) specifically detects Histone H4 in WB, IF, ICC, ChIP, DB and ChIP-seq. The antibody is reactive with Human, Mouse and Saccharomyces cerevisiae samples.

Quick Overview for Histone H4 antibody (H4K12ac) (ABIN6579166)

Target: Histone H4 (HISTH4)

Reactivity: Human, Mouse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone H4 Antibody

Binding Specificity: H4K12ac

Purpose: Histone H4K12ac antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H4 acetyl Lys12 antibody was raised against a peptide including acetyl-lysine 12 of histone H4.

Application Details

Application Notes: ChIP: 2 - 8 µL per ChIP ChIP-Seq: 5 µL each ICC/IF: 1:500 - 1:2,000 dilution WB: 1:5,000 - 1:20,000 dilution The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H4 acetyl Lys12 antibody was validated for ChIP-Seq in the study (see reference).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H4 antibody (ABIN6579166)

Meers, Henriques, Lavender, McKay, Strahl, Duronio, Adelman, Matera: "Histone gene replacement reveals a post-transcriptional role for H3K36 in maintaining metazoan transcriptome fidelity." in: eLife, Vol. 6, (2017) (PubMed).

Johnson, French, Osheim, Li, Hall, Beyer, Smith: "Rpd3- and spt16-mediated nucleosome assembly and transcriptional regulation on yeast ribosomal DNA genes." in: Molecular and cellular biology, Vol. 33, Issue 14, pp. 2748-59, (2013) (PubMed).

Wan, Hu, Liu, Han, Sood, Calin, Zhang, Lu: "A novel non-coding RNA lncRNA-JADE connects DNA damage signalling to histone H4 acetylation." in: The EMBO journal, Vol. 32, Issue 21, pp. 2833-47, (2013) (PubMed).

Larsson, Ulfhammer, Magnusson, Bergh, Lunke, El-Osta, Medcalf, Svensson, Karlsson, Jern: "Role of histone acetylation in the stimulatory effect of valproic acid on vascular endothelial tissue-type plasminogen activator expression." in: PLoS ONE, Vol. 7, Issue 2, pp. e31573, (2012) (PubMed).

Liu, McConnell, Dixon, Calvi: "Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex." in: Molecular biology of the cell, Vol. 23, Issue 1, pp. 200-12, (2012) (PubMed).

Silva, Xu, Kim, Fillingham, Kislinger, Mennella, Keogh: "The replication-independent histone H3-H4 chaperones HIR, ASF1, and RTT106 co-operate to maintain promoter fidelity." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 1709-18, (2012) (PubMed).

Li, Corsa, Pan, Wu, Ferguson, Yu, Min, Dou: "MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1." in: Molecular and cellular biology, Vol. 30, Issue 22, pp. 5335-47, (2010) (PubMed).

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The acetylation of histone H4 Lys12 is carried out by several histone acetyltransferases (HATs). Histone H4 Lys12 acetylation by TIP60 occurs in the nucleus, whereas Hat1-mediated acetylation of histone H4 Lys12 occurs in the cytoplasm, indicating the spatial regulation of histone acetylation. The acetylation of histone H4 Lys12 residue is commonly associated with transcriptional activation.

Molecular Weight: 8 kDa

Gene ID: 121504

NCBI Accession: NP_778224