L3MBTL1 antibody (AA 1-215)

Catalog No. ABIN6579167

There are 2+ publications for this product available. The Rabbit Polyclonal anti-L3MBTL1 antibody is suitable to detect L3MBTL1 in samples from Human. It has been validated for WB, ChIP and ChIP-seq.

Quick Overview for L3MBTL1 antibody (AA 1-215) (ABIN6579167)

Target: L3MBTL1 (L(3)mbt-Like 1 (L3MBTL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This L3MBTL1 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-L3MBTL1 Antibody

Binding Specificity: AA 1-215

Purpose: L3MBTL1 antibody (pAb)

Purification: Unpurified

Immunogen: This L3MBTL1 antibody was raised against a His-Tagged fusion protein corresponding to amino acids 1-215 of human L3MBTL1.

Application Details

Application Notes: WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-L3MBTL1 antibody (ABIN6579167)

Qin, Van Buren, Huang, Zhong, Mostoslavsky, Akbarian, Hock: "Chromatin protein L3MBTL1 is dispensable for development and tumor suppression in mice." in: The Journal of biological chemistry, Vol. 285, Issue 36, pp. 27767-75, (2010) (PubMed).

Qi, Sarkissian, Hu, Wang, Bhattacharjee, Gordon, Gonzales, Lan, Ongusaha, Huarte, Yaghi, Lim, Garcia, Brizuela, Zhao, Roberts, Shi: "Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development." in: Nature, Vol. 466, Issue 7305, pp. 503-7, (2010) (PubMed).

Target Details for L3MBTL1

Target: L3MBTL1 (L(3)mbt-Like 1 (L3MBTL1))

Alternative Name: L3MBTL1

Background: L3MBTL1 is a human homolog of the Drosophila l(3)mbt protein. Located on chromosome 20q12, L3MBT1 is a member of the malignant brain tumor (MBT) protein family and directly compacts chromatin in a strictly histone methylation dependent fashion. L3MBTL1 functions as a transcriptional repressor in a manner that requires its three MBT domains, which were predicted to bind methylated lysines of histones. L3MBTL1 binds to Lys20 of Histone H4 that has been mono- or dimethylated, as well as to Histone H1 isotype b methylated at lysine 26. L3MBTL1 is a complex molecule with a Zinc-finger and a sterile alpha motif (SAM).

Molecular Weight: 100 kDa

Gene ID: 26013

NCBI Accession: NP_056293

Pathways: Chromatin Binding