HLA-DQ1/DQ2 antibody (AA 131-180)

Catalog No. ABIN6579687

The Rabbit Polyclonal anti-HLA-DQ1/DQ2 antibody is suitable to detect HLA-DQ1/DQ2 in samples from Human. It has been validated for ELISA, WB, IF and IHC.

Quick Overview for HLA-DQ1/DQ2 antibody (AA 131-180) (ABIN6579687)

Target: HLA-DQ1/DQ2 (MHC Class II HLA-DQ1/DQ2 (HLA-DQ1/DQ2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DQ1/DQ2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-HLA-DQ1/DQ2 Antibody

Binding Specificity: AA 131-180

Purpose: HLA-DQB1/2 Antibody

Specificity: HLA-DQB1/2 Polyclonal Antibody detects endogenous levels of HLA-DQB1/2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human HLA-DQB1/HLA-DQB2. AA range:131-180

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HLA-DQ1/DQ2

Target: HLA-DQ1/DQ2 (MHC Class II HLA-DQ1/DQ2 (HLA-DQ1/DQ2))

Alternative Name: HLA-DQB1/2

Background:

Synonyms: HLA-DQB1, HLA-DQB, HLA class II histocompatibility antigen, DQ beta 1 chain, MHC class II antigen DQB1, HLA-DQB2, HLA-DXB, HLA class II histocompatibility antigen, DQ beta 2 chainHLA class II histocompatibility antigen, DX beta chain, MHC class II antigen DQB2

Background: major histocompatibility complex, class II, DQ beta 1(HLA-DQB1) Homo sapiens HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marro

Gene Name: HLA-DQB1/HLA-DQB2

Protein Name: HLA class II histocompatibility antigen DQ beta 1 chain/HLA class II histocompatibility antigen DQ beta 2 chain

Molecular Weight: 29 kDa

Gene ID: 100507714

UniProt: P01920