EIF5A antibody (acLys47)

Catalog No. ABIN6579717

This Rabbit Polyclonal antibody specifically detects EIF5A in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EIF5A antibody (acLys47) (ABIN6579717)

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EIF5A Antibody

Binding Specificity: AA 11-60, acLys47

Purpose: Acetyl eIF5A/eIF5A2 (K47) Antibody

Specificity: Acetyl-eIF5A/eIF5A2 (K47) Polyclonal Antibody detects endogenous levels of eIF5A/eIF5A2 protein only when acetylated at K47.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47. AA range:11-60

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for EIF5A

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Alternative Name: eIF5A

Background:

Synonyms: EIF5A2, Eukaryotic translation initiation factor 5A-2, eIF-5A-2, eIF-5A2, Eukaryotic initiation factor 5A isoform 2

Background: function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.,

Gene Name: EIF5A2

Protein Name: Eukaryotic translation initiation factor 5A-2

Molecular Weight: 16 kDa

Gene ID: 56648, 56648

OMIM: 600187

UniProt: Q9GZV4

Pathways: Regulation of Muscle Cell Differentiation