Hsc70 antibody

Catalog No. ABIN6653942

This Rat Monoclonal antibody specifically detects Hsc70 in WB, IHC, IP and ICC. It exhibits reactivity toward Human, Rat, Mouse, Chicken and Mammalian.

Quick Overview for Hsc70 antibody (ABIN6653942)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human, Rat, Mouse, Chicken, Mammalian

Host: Rat

Clonality: Monoclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 1B5

Product Details anti-Hsc70 Antibody

Specificity: Reacts with human Hsc70 (Hsp73). Cross-reacts with mouse, rat, rabbit, guinea pig, hamster, monkey, sheep, chicken, bovine, canine and porcine protein.

Purification: Purified (protein G)

Immunogen: Hsc70 purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.

Isotype: IgG2a

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB0.1 μg/mL IP5 μg/mL IHC5 μg/mL ICC5 μg/mL

Positive control: HeLa Heat Shocked Cell Lysate

Restrictions: For Research Use only

Handling

Buffer: PBS pH 7.2, 0.1 mM PMSF in 50 % glycerol.

Preservative: PMSF

Storage: 4 °C/-20 °C

Storage Comment: Store frozen product at or below -20°C. Thawed product may be stored for 2-4 weeks at 4°C. For optimal storage, aliquot and store at -20°C.

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: Hsc70 (Hsp73)

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes

Heat shock cognate 71 kDa protein Heat shock 70 kDa protein 8 Lipopolysaccharide-associated protein 1, LAP-1, LPS-associated protein 1 Gene name: HSPA8, HSC70, HSP73, HSPA10

Gene ID: 3312

UniProt: P11142