AMACR antibody (AA 297-394)

Catalog No. ABIN6654230

This anti-AMACR antibody is a Rabbit Monoclonal antibody detecting AMACR in IHC (p). Suitable for Human.

Quick Overview for AMACR antibody (AA 297-394) (ABIN6654230)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AMACR-2748R

Product Details anti-AMACR Antibody

Binding Specificity: AA 297-394

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A portion of amino acids 297-394 was used as the immunogen for the recombinant AMACR antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant AMACR antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant AMACR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: AMACR / p504S

Background: This antibody recognizes a protein of 43 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Pathways: Monocarboxylic Acid Catabolic Process