Recombinant Mitochondrial Marker antibody

Catalog No. ABIN6654364

The Rabbit Monoclonal anti-Mitochondrial Marker antibody is suitable to detect Mitochondrial Marker in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant Mitochondrial Marker antibody (ABIN6654364)

Target: Mitochondrial Marker

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: MTC02-2860R

Product Details

Purpose: Recombinant Mitochondrial Marker Antibody (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: A semi-purified mitochondria preparation was used as the immunogen for this recombinant Mitochondrial Marker antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Mitochondrial Marker antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Mitochondrial Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Mitochondrial Marker

Background: This MAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors.