Recombinant BrdU antibody

Catalog No. ABIN6654471

This Rabbit Monoclonal antibody specifically detects BrdU in IHC (p).

Quick Overview for Recombinant BrdU antibody (ABIN6654471)

Target: BrdU (Bromodeoxyuridine (BrdU))

Antibody Type: Recombinant Antibody

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BRD2888R

Product Details anti-BrdU Antibody

Purpose: BrdU Antibody / Bromodeoxyuridine

Purification: Protein A affinity chromatography

Immunogen: BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this BrdU antibody.

Isotype: IgG

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical

Background: This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.