Fibronectin antibody (N-Term)

Catalog No. ABIN6654476

This anti-Fibronectin antibody is a Mouse Monoclonal antibody detecting Fibronectin in IHC (p). Suitable for Human.

Quick Overview for Fibronectin antibody (N-Term) (ABIN6654476)

Target: Fibronectin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 616

Product Details anti-Fibronectin Antibody

Binding Specificity: N-Term

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: High molecular weight proteins secreted by cultivated human fibroblasts were used as the immunogen for the Fibronectin antibody. The epitope of this mAb is located in the 29kDa N-terminal fibrin- and heparin-binding domain of fibronectin.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the Fibronectin antibody should be determined by the researcher.

1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb does not cross-react with MMP-2, MMP-9, or TIMP-2.