Desmin antibody

Catalog No. ABIN6654512

This anti-Desmin antibody is a Rabbit Monoclonal antibody detecting Desmin in IHC (p). Suitable for Human.

Quick Overview for Desmin antibody (ABIN6654512)

Target: Desmin (DES)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DES-2960R

Product Details anti-Desmin Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the recombinant Desmin antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Desmin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Desmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin

Background: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.