ANO1 antibody (AA 2-101)

Catalog No. ABIN6654568

The Rabbit Monoclonal anti-ANO1 antibody has been validated for IHC (p). It is suitable to detect ANO1 in samples from Human.

Quick Overview for ANO1 antibody (AA 2-101) (ABIN6654568)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DG1-2564R

Product Details anti-ANO1 Antibody

Binding Specificity: AA 2-101

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A portion of amino acids 2-101 from the human protein was used as the immunogen for this recombinant DOG1 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant DOG1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant DOG1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: DOG1 / TMEM16A / ANO1

Background: Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST's is nearly identical: 94.4 % vs. 94.7 %.