BAP1 antibody (AA 191-326)

Catalog No. ABIN6654721

The Mouse Monoclonal anti-BAP1 antibody has been validated for IHC (p). It is suitable to detect BAP1 in samples from Human.

Quick Overview for BAP1 antibody (AA 191-326) (ABIN6654721)

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAP1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BAP1-2431

Product Details anti-BAP1 Antibody

Binding Specificity: AA 191-326

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: A portion of amino acids 191-326 from the human protein was used as the immunogen for the BAP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the BAP1 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the BAP1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for BAP1

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Alternative Name: BAP1 / BRCA1-Associated Protein 1

Background: The BAP1 gene belongs to the ubiquitin C-terminal hydrolase subfamily of de-ubiquitination enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germ line mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, Uveal melanoma and cutaneous melanoma.