Ki-67 antibody (AA 2293-2478)

Catalog No. ABIN6654767

This anti-Ki-67 antibody is a Mouse Monoclonal antibody detecting Ki-67 in FACS, IF, ICC and IHC (p). Suitable for Human.

Quick Overview for Ki-67 antibody (AA 2293-2478) (ABIN6654767)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MKI67-2466

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 2293-2478

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: A portion of amino acids 2293-2478 was used as the immunogen for the Ki-67 antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the Ki-67 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 1-2 μg/10^6 cells in 0.1ml,IF/ICC: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Ki-67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki67

Background: Ki67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki67 index and the histo-pathological grade of neoplasms. Assessment of Ki67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki67 is a good marker of proliferating cell populations.

Pathways: Glycosaminoglycan Metabolic Process