MTAP antibody (AA 97-196)

Catalog No. ABIN6654904

The Rabbit Monoclonal anti-MTAP antibody has been validated for WB and IHC (p). It is suitable to detect MTAP in samples from Human.

Quick Overview for MTAP antibody (AA 97-196) (ABIN6654904)

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MTAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SMTP-1R

Product Details anti-MTAP Antibody

Binding Specificity: AA 97-196

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A portion of amino acids 97-196 from the human protein was used as the immunogen for the recombinant MTAP antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant MTAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant MTAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Alternative Name: MTAP

Background: Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. [UniProt]

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process