MUC3A antibody

Catalog No. ABIN6654972

This Rabbit Monoclonal antibody specifically detects MUC3A in IHC (p). It exhibits reactivity toward Human.

Quick Overview for MUC3A antibody (ABIN6654972)

Target: MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MUC3A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MUC3-2992R

Product Details anti-MUC3A Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the MUC3 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant MUC3 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant MUC3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MUC3A

Target: MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

Alternative Name: MUC3

Background: It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.