Elastase 3B antibody (AA 82-238)

Catalog No. ABIN6655004

This anti-Elastase 3B antibody is a Rabbit Monoclonal antibody detecting Elastase 3B in IHC (p). Suitable for Human.

Quick Overview for Elastase 3B antibody (AA 82-238) (ABIN6655004)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Elastase 3B antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CELA3B-2809R

Product Details anti-Elastase 3B Antibody

Binding Specificity: AA 82-238

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A recombinant partial protein (aa 82-238) was used as the immunogen for the recombinant CELA3B antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant CELA3B antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CELA3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: CELA3B / Elastase 3B

Background: This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function.