Acetylated Lysine antibody

Catalog No. ABIN6655030

Supplier Product No.: 200-301-a97

The Mouse Monoclonal anti-Acetylated Lysine antibody is suitable to detect Acetylated Lysine. It has been validated for WB, ELISA and IHC.

Quick Overview for Acetylated Lysine antibody (ABIN6655030)

Target: Acetylated Lysine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Acetylated Lysine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 7F8

Product Details anti-Acetylated Lysine Antibody

Supplier Product No.: 200-301-a97

Supplier: Rockland

Purpose: Lysine Acetylated Antibody

Cross-Reactivity (Details): This antibody is known to react with acetylated lysine residues from mammalian and avian sources.

Purification: This Protein G purified monoclonal antibody reacts with proteins containing acetylated lysine residues and does not detect non-acetylated lysine residues.

Sterility: Sterile filtered

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with acetylated keyhole limpet hemocyanin (KLH).

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Comment:

This Protein G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Approximately 1 μg of antibody is sufficient to detect 20 μg of acetylated chicken erythrocyte histones (sodium butyrate treated) by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110). Antibody provided in PBS pH 7.4.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Acetylated Lysine

Target: Acetylated Lysine

Alternative Name: Lysine Acetylated

Target Type: Chemical

Background:

Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. A common posttranscriptional modification of lysine residues involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4). HDACs therefore are promising targets for anticancer drugs currently in development (5).