Rho-related GTP-binding protein antibody

Catalog No. ABIN6655039

Supplier Product No.: 200-301-e29

The Mouse Monoclonal anti-Rho-related GTP-binding protein antibody has been validated for WB and IHC. It is suitable to detect Rho-related GTP-binding protein in samples from Cow.

Quick Overview for Rho-related GTP-binding protein antibody (ABIN6655039)

Target: Rho-related GTP-binding protein (RhO (pan))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Rho-related GTP-binding protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 1D4

Product Details anti-Rho-related GTP-binding protein Antibody

Supplier Product No.: 200-301-e29

Supplier: Rockland

Purpose: Rhodopsin Antibody

Cross-Reactivity (Details): Anti-Rhodopsin antibody is directed against bovine Rhodopsin.

Purification: Rhodopsin antibodies are Protein G purified from cell culture supernantant.

Immunogen: Anti-Rhodopsin Monoclonal Antibody was produced in mouse by repeated immunizations with purified native bovine rhodopsin.

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-Rhodopsin antibody has been tested in Western Blot and Immunohistochemistry. Expect a band of approximately 39 kDa in size corresponding to the rhodopsin proteins in Western blot in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Rho-related GTP-binding protein

Target: Rho-related GTP-binding protein (RhO (pan))

Alternative Name: RHO

Background:

Synonyms: OPN2, Rhodopsin, RHO, Anti-Rhodopsin monoclonal, photoreceptor proteins

Background: Rhodopsin antibody detects rhodopsin which is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis. Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin. Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding. Anti-Rhodopsin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: RHO

Gene ID: 509933

UniProt: P02699