MAP2 antibody

Catalog No. ABIN6655044

Supplier Product No.: 200-901-d68

Product Details anti-MAP2 Antibody

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Cow

Host: Chicken

Clonality: Polyclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 200-901-d68

Supplier: Rockland

Purpose: Microtubule Associated Protein 2 (MAP2) Antibody

Cross-Reactivity (Details): Anti-MAP2 antibody is directed against MAP2 protein.

Purification: The antibody is a total IgY fraction.

Immunogen: Anti-Microtubule Associated Protein 2 Antibody was produced in chicken by repeated immunizations with recombinant bovine MAP2 protein expressed in and purified from E. Coli.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:2500

Western_Blot_Dilution: 1:20000

Other: User Optimized

Comment:

Anti-Microtubule Associated Protein 2 antibody is tested for use in Western Blotting, ICC, and IHC. Specific conditions should be optimized by the end user. Expect a band of approximately 280 kDa in size corresponding to MAP2 proteins in Western blots in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: Microtubule Associated Protein 2 (MAP2) (MAP2 Products)

Background:

Synonyms: Microtubule-associated protein 2, MAP2, MAP-2,Map2, Map-2

Background: MAP2 antibody detects Microtubule Associated Protein 2. Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone.

Gene Name: MAP2

Gene ID: 4133

UniProt: P11137