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Spectrin, Alpha, beta antibody

Reactivity: Human WB, ELISA Host: Mouse Monoclonal Spe 1-2 unconjugated
Catalog No. ABIN6655089
  • Target
    Spectrin, Alpha, beta
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Western Blotting (WB), ELISA
    Cross-Reactivity (Details)
    Cross reactivity with Spectrin from other sources has not been determined.
    Purification
    Anti-Spectrin antibody was purified from mouse ascites by protein A chromatography.  This product reacts with human.
    Immunogen

    Immunogen: Spectrin(alpha and beta) antibody was produced in mice by repeated immunizations with human erythrocyte spectrin.

    Immunogen Type: Other

    Clone
    Spe 1-2
    Isotype
    IgG1
  • Application Notes

    Application Note: Anti-Spectrin is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~246-280 kDa corresponding to the appropriate cell lysate or extract.

    ELISA Dilution: 1:40,000

    Western Blot Dilution: 2-4 μg/mL

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Neutral PBS

    Buffer

    Buffer: 1.2 % sodium acetate

    Stabilizer: 2 mg BSA

    0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    Spectrin, Alpha, beta
    Alternative Name
    Spectrin (alpha and beta)
    Background

    Synonyms: EL2, HPP, HS3, SPH3, SPTA, EL3, HS2, SPH2, HSPTB1, Erythroid alpha-spectrin

    Background: Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5. This antibody is suitable for researchers interested in cytoskeleton and transcriptoion factor research.

    Gene Name: SPTA1, SPTB

    Gene ID
    6708
    NCBI Accession
    NP_003117
    UniProt
    P02549
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