CD40 Ligand antibody (PE)

Catalog No. ABIN6655108

Supplier Product No.: 200-308-n76

The Mouse Monoclonal anti-CD40 Ligand antibody has been validated for FACS and FM. It is suitable to detect CD40 Ligand in samples from Human.

Quick Overview for CD40 Ligand antibody (PE) (ABIN6655108)

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is conjugated to PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: 24-31

Product Details anti-CD40 Ligand Antibody (PE)

Supplier Product No.: 200-308-n76

Supplier: Rockland

Purpose: CD154 (CD40L) Phycoerythrin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD154.

Purification: Phycoerythrin conjugated CD154 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD154.

Sterility: Sterile filtered

Immunogen: Anti-CD154 Antibody (Monoclonal) was produced by repeated immunizations with CD154 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD154 is tested for Flow Cytometry and useful in Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 (CD40L)

Background:

Synonyms: CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD40LG, CD40L, TNFSF5, TRAP

Background: CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Gene Name: CD40LG

Gene ID: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints