Integrin beta 3 antibody (C-Term)

Catalog No. ABIN6655110

Supplier Product No.: 600-401-gu0s

This Rabbit Polyclonal antibody specifically detects Integrin beta 3 in WB, IHC, FACS, ELISA, IP and FM. It exhibits reactivity toward Human.

Quick Overview for Integrin beta 3 antibody (C-Term) (ABIN6655110)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Product Details anti-Integrin beta 3 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-gu0s

Supplier: Rockland

Purpose: Integrin beta 3 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Integrin beta 3.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Integrin beta 3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human Integrin beta 3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:100 - 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: Not recommended

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-Integrin beta-3 Antibody has been tested in Western Blot, ELISA and IHC. Positive control lysates used U-87 p/n (W09-001-GX2) and MDA-MB-435 p/n (W09-001-A39) @ 1μg/mL in WB. Expect possible bands at ~80, 100 and 130 kDa in western blot using appropriate lysates. Immunohistochemistry application with human breast carcinoma tissue is recommended. Although not tested, this antibody is likely functional in flow cytometry and immunoprecipitation. This antibody is not recommended in immunofluorescence detection.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: Integrin beta 3

Background:

Synonyms: rabbit anti-Integrin beta 3 antibody, Integrin Subunit beta 3, IGTB3, GP3A, Platelet membrane glycoprotein IIIa, GPIIIa, Integrin beta-3, CD61, CD61 Antibody

Background: Integrin beta 3, also called ITB3, belongs to the integrin beta chain family. Integrin alpha-V/beta-3 (ITGAV/ITB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B/ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. ITGA2B/ITGB3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. ITGAV/ITB3 binds to CX3CL1, NRG1, FGF1, IGF1, PLA2G2A, and FBN1. ITGAV/ITGB3 acts as a receptor for herpes virus 8/HHV-8, coxsackievirus A9, Hantaan virus, cytomegalovirus/HHV-5, human metapneumovirus, human parechovirus 1, and west nile virus. Intergrin beta 3 may be associated with disorders such as Glanzmann thrombasthenia, myocardial infarction, purpura, and insulin-like growth factor i deficency. Anti-Integrin beta 3 Antibody is useful for researched interested in RET Signaling, MAPK-Erk Pathway, ERK Signaling, and Integrin Pathway Research.

Gene Name: ITGB3

Gene ID: 3690

NCBI Accession: NP_000203

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex