SQSTM1 antibody (pSer272, pThr269)

Catalog No. ABIN6655165

Supplier Product No.: 600-401-x19

The Rabbit Polyclonal anti-SQSTM1 antibody has been validated for WB and ELISA. It is suitable to detect SQSTM1 in samples from Human.

Quick Overview for SQSTM1 antibody (pSer272, pThr269) (ABIN6655165)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SQSTM1 Antibody

Binding Specificity: pSer272, pThr269

Supplier Product No.: 600-401-x19

Supplier: Rockland

Purpose: p62 Phospho T269/Ser272 Antibody

Cross-Reactivity (Details): Anti-Phospho-Thr269/Ser272 p62 Antibody is directed against p62 protein phosphorylated at T269 and S272.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Thr269/Ser272 p62 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Thr269/Ser272 of human p62 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Thr269/Ser272 p62 Antibody is tested for Western Blots and is specific for the ~62 kDa p62 protein phosphorylated at Thr269 and Ser272. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background:

Synonyms: Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62

Background: p62, also known as sequestosome1 (SQSTM1), is a shuttle protein transporting polyubiquitinated proteins for both proteasomal and lysosomal degradation. p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease. p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages. Phosphorylation of Ser28 has recently been demonstrated to be related to the pathogenesis of Parkinson's disease. p62 pT269/pS272 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: SQSTM1

Gene ID: 8878

NCBI Accession: NP_003891

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy