Progesterone Receptor antibody (pSer294)

Catalog No. ABIN6655216

Supplier Product No.: 209-301-e17

The Mouse Monoclonal anti-Progesterone Receptor antibody has been validated for WB and IHC. It is suitable to detect Progesterone Receptor in samples from Human.

Quick Overview for Progesterone Receptor antibody (pSer294) (ABIN6655216)

Target: Progesterone Receptor (PGR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Progesterone Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 608

Product Details anti-Progesterone Receptor Antibody

Binding Specificity: pSer294

Supplier Product No.: 209-301-e17

Supplier: Rockland

Purpose: Progesterone Receptor phospho S294 Antibody

Purification: Anti-Progesterone Receptor pS294 (Mouse) Monoclonal Antibody is Protein G purified from cell culture supernatant. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Immunogen: Anti-Progesterone Receptor pS294 (Mouse) Monoclonal Antibody was produced in mouse by repeated immunizations with Progesterone Receptor pS294- Synthetic peptide corresponding to amino acid residues surrounding Ser294 of the human progesterone receptor protein.

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Progesterone Receptor pS294 (Mouse) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should but optimized by the end user. Expect a band of approximately 90 kDa in size for PR-A isoform and the approximately 120 kDa PR-B isoform phosphorylated at Ser294.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Progesterone Receptor

Target: Progesterone Receptor (PGR)

Alternative Name: Progesterone Receptor

Background:

Synonyms: mouse anti-Progesterone Receptor phospho S294 Antibody, mouse anti-Progesterone Receptor pS294 antibody, PR, Nuclear receptor subfamily 3 group C member 3

Background: There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor. Anti-Progesterone Receptor pS294 (Mouse) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Cancer research.

Gene Name: PGR

Gene ID: 5241

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration