Thrombospondin 2 antibody (Internal Region)

Catalog No. ABIN6655233

Supplier Product No.: 600-401-r01

This Rabbit Polyclonal antibody specifically detects Thrombospondin 2 in WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Thrombospondin 2 antibody (Internal Region) (ABIN6655233)

Target: Thrombospondin 2 (THBS2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thrombospondin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Thrombospondin 2 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-r01

Supplier: Rockland

Purpose: THBS2 Antibody

Cross-Reactivity (Details): Anti-THBS2 antibody is directed against human THBS2 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: THBS2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human THBS2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

Flow_Cytometry_Dilution: 1-3 μg/1x106 cells

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: ICC: 0.5-1 μg/mL

Comment:

Anti-THBS2 is tested for Flow Cytometry, Immunohistochemistry-P, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~130 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Thrombospondin 2

Target: Thrombospondin 2 (THBS2)

Alternative Name: THBS2

Background:

Synonyms: THBS2, Thrombospondin-2, TSP2

Background: Thrombospondin-2 (THBS2) is a protein that in humans is encoded by the THBS2 gene. The protein encoded by this gene belongs to the thrombospondin family. The THBS2 is mapped to 6q27 and it is located on chromosome 17. The gene was transcribed in fibroblasts, smooth muscle cells, and an osteosarcoma cell line. It functions as a protein inhibitor of tumor growth and angiogenesis and modulates the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. This antibody is suitable for researchers interested in cancer research.

Gene Name: THBS2

Gene ID: 7058

NCBI Accession: NP_003238

UniProt: P35442