Cytokeratin 7 antibody

Catalog No. ABIN6655254

Supplier Product No.: 200-301-p34

This Mouse Monoclonal antibody specifically detects Cytokeratin 7 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 7 antibody (ABIN6655254)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: LDS-68

Product Details anti-Cytokeratin 7 Antibody

Supplier Product No.: 200-301-p34

Supplier: Rockland

Purpose: Cytokeratin Peptide 7 Antibody

Cross-Reactivity (Details): This product reacts with human.

Purification: Anti-Cytokeratin Peptide 7 antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Cytokeratin Peptide 7 antibody was produced in mice by repeated immunizations with cytoskeletal preparation of the RT4 human bladder carcinoma cell line.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 1-2 μg/mL

Comment:

Anti-Cytokeratin Peptide 7 is tested for Immunohistochemistry -P, -F, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~51 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Cytokeratin Peptide 7

Background:

Synonyms: SCL, cytokeratin 7, K2C7, K7, keratin, Keratin-7, 55K type II cytoskeletal, keratin, type II cytoskeletal 7, sarcolectin, , KERATIN, SIMPLE EPITHELIAL, KERATIN, TYPE II, CYTOSKELETAL, 7, SARCOLECTIN, Type-II keratin Kb7

Background: KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73 % homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans. This antibody is suitable for researchers interested in cancer research, cardiovascular diseases, and cytoskeletal signaling research.

Gene Name: KRT7

Gene ID: 3855

NCBI Accession: NP_005547

UniProt: P08729