MAPK14 antibody

Catalog No. ABIN6655303

Supplier Product No.: 200-301-g27

The Mouse Monoclonal anti-MAPK14 antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect MAPK14 in samples from Human.

Quick Overview for MAPK14 antibody (ABIN6655303)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: 9F12

Product Details anti-MAPK14 Antibody

Supplier Product No.: 200-301-g27

Supplier: Rockland

Purpose: p38 alpha MAPKinase Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest no known cross-reactivity with other related human kinases.

Purification: Anti- p38 α MAP Kinase Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: p38 Antibody was produced in mice by repeated immunizations raised against a full length recombinant protein expressed in E.coli cells.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:1000

Comment:

Anti-p38 MAP kinase Antibody is tested for use in WB, IP, IHC, and ELISA. Expect a band approximately ~38kDa protein corresponding to the molecular mass of p38α MAPK on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: p38 alpha MAPKinase

Background:

Synonyms: CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A, Mitogen-activated protein kinase 14, Cytokine suppressive anti-inflammatory drug-binding protein

Background: The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway. The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death. Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP. p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs. As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis and has also been implicated in cancer, CNS and diabetes.

Gene Name: MARK14

Gene ID: 1432

NCBI Accession: NP_001306

UniProt: Q16539

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins