MITF antibody

Catalog No. ABIN6655359

Supplier Product No.: 200-301-h93

This Mouse Monoclonal antibody specifically detects MITF in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for MITF antibody (ABIN6655359)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 21D1418

Product Details anti-MITF Antibody

Supplier Product No.: 200-301-h93

Supplier: Rockland

Purpose: MITF Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with MITF from Human, Rat, and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-MITF Antibody was purified by Protein G chromatography.

Immunogen: MITF Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide (sequence PSTGLCSPDLVN) corresponding to internal amino acids with human MITF protein.

Isotype: IgG1

Application Details

Application Notes: Optional[Neutralization_Dilution]: 3-5 μg/mL

Comment:

Anti-MITF antibody is tested for WB, IHC, and IF. Expect a band approximately 52kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background:

Synonyms: BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32

Background: Anti-MITF antibody detects human MITF. Microphthalmia-associated bHLH-LZ transcription factor (MITF) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC. MITF can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. MITF is expressed as multiple isoforms, which share common activation and DNA-binding domains, but differ in the amino-terminal region. MITF is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases. Anti-MITF antibody is ideal for investigators involved in kinase, phosphatase, NFkappaB, and signaling research.

Gene Name: MITF

Gene ID: 4286

NCBI Accession: NP_000239

UniProt: O75030

Pathways: Chromatin Binding