CTSA antibody

Catalog No. ABIN6655368

Product Details anti-CTSA Antibody

Target: CTSA (Cathepsin A (CTSA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTSA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: This antiserum is directed against PPCA and reacts with the PPCA from human tissues.  Based on sequence we expect this antibody to react with PPCA from other sources, although specific reactivity has not been confirmed.

Immunogen:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with native 54-kDa human Protective Protein/Cathepsin A (PPCA) precursor, purified from the medium of baculovirus infected insect cells.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500

Application Note: This antibody is suitable for the detection of human PPCA (54-, 32-, and 20- kDa) on Western blot, by immunoprecipitation, immunocytochemistry, and ELISA of either transfected cells (e.g. COS-1 cells) or primary cells (e.g. fibroblasts).

ELISA Dilution: 1:1,000 - 1:5,000

Western Blot Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.1 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for CTSA

Target: CTSA (Cathepsin A (CTSA))

Alternative Name: Protective Protein/Cathepsin A (CTSA Products)

Synonyms: Ppgb antibody, beta-galactosidase 2 antibody, PPGB antibody, GLB2 antibody, GSL antibody, NGBE antibody, PPCA antibody, AU019505 antibody, cathepsin A antibody, beta-galactosidase 2 antibody, Ctsa antibody, BGAL2 antibody, CTSA antibody

Background:

Synonyms: rabbit anti-Protective Protein Antibody, rabbit anti-Cathepsin A Antibody, Beta Galactosidase Protective Protein antibody, Carboxypeptidase C antibody, Cathepsin A antibody, CTSA antibody, Glactosialidosis antibody, GLB2 antibody, Goldberg Syndrome antibody

Background: Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54- kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20- kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH . Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Gene Name: CTSA, PPGB

Gene ID: 5476

UniProt: P10619