TRAF6 antibody

Catalog No. ABIN6655371

Supplier Product No.: 200-401-i32

This Rabbit Polyclonal antibody specifically detects TRAF6 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for TRAF6 antibody (ABIN6655371)

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-TRAF6 Antibody

Supplier Product No.: 200-401-i32

Supplier: Rockland

Purpose: TRAF6 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TRAF6 from human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TRAF6 Antibody was purified by Protein G chromatography.

Immunogen: TRAF6 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to an internal amino acid sequence in the human protein TRAF6.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 3-5 μg/mL

Comment:

Anti-TRAF6 Antibody is tested for use in WB and FLOW. Expect a band approximately 54kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TRAF6

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Alternative Name: TRAF6

Background:

Synonyms: RNF85, NF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85

Background: Anti-TRAF6 Antibody detects human TRAF6. Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). Recently it has been shown that TRANCE/OPGL activates the antiapoptotic serine/threonine kinase Akt/PKB through a signaling complex involving c-Src and TRAF6. Mice deficient in TNF receptor-associated factor 6 (TRAF6) are osteopetrotic with defects in bone remodeling and tooth eruption due to impaired osteoclast function. Like TRAF2 and TRAF3, TRAF6 is also essential for perinatal and postnatal survival. These findings establish unexpectedly diverse and critical roles for TRAF6 in perinatal and postnatal survival, bone metabolism, LPS, and cytokine signaling. RANK (TRANCE receptor) interacts with various TRAFs through distinct motifs and activates NF-kB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kB activation. Over-expression of TRAF6 activates JNK, p38, or IKK in the absence of extracellular stimulation. Anti-TRAF6 Antibody is ideal for investigators involved in Toll Like Receptors and signaling, cyotkines, growth factors and AKT Singaling.

Gene Name: TRAF6

Gene ID: 7189

NCBI Accession: NP_004611

UniProt: Q9Y4K3

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway