Beclin 1 antibody (pSer234)

Catalog No. ABIN6655419

Supplier Product No.: 600-401-w96

This Rabbit Polyclonal antibody specifically detects Beclin 1 in WB and ELISA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Beclin 1 antibody (pSer234) (ABIN6655419)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Beclin 1 Antibody

Binding Specificity: pSer234

Supplier Product No.: 600-401-w96

Supplier: Rockland

Purpose: BECLIN1 phospho S234 Antibody

Cross-Reactivity (Details): Anti-Beclin1 pS234 Antibody is directed against Beclin 1 protein phosphorylated at S234.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser234 Beclin-1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of human Beclin-1 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:2,500

Western_Blot_Dilution: 1:250

Comment:

Suggested Applications: ELISA
Anti-Phospho-Ser234 Beclin-1 Antibody is tested for WB. Expect a band at approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user. Immunolabeling of the Beclin-1 band is decreased in the Beclin-1 alanine substitution mutant.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Beclin 1 antibody (ABIN6655419)

Xie, Kang, Tang: "Assessment of Posttranslational Modifications of ATG proteins." in: Methods in enzymology, Vol. 587, pp. 171-188, (2017) (PubMed).

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin1

Background:

Synonyms: Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein

Background: Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies. Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234. Beclin 1 pS234 Antibody is ideal for researchers interested in cancer research.

Gene Name: BECN1

Gene ID: 8678

NCBI Accession: NP_001300927

UniProt: Q14457

Pathways: Autophagy