CD19 antibody (FITC)

Catalog No. ABIN6655429

Supplier Product No.: 200-302-n24

The Mouse Monoclonal anti-CD19 antibody has been validated for FACS. It is suitable to detect CD19 in samples from Human. There is 1 publication available.

Quick Overview for CD19 antibody (FITC) (ABIN6655429)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: HIB19

Product Details anti-CD19 Antibody (FITC)

Supplier Product No.: 200-302-n24

Supplier: Rockland

Purpose: CD19 Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD19 and chimpanzee.

Purification: Fluorescein conjugated CD19 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD19.

Sterility: Sterile filtered

Immunogen: Anti-CD19 Antibody (Monoclonal) was produced by repeated immunizations with CD19 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 4-6

Application Details

Application Notes: Optional[Neutralization_Dilution]: 5 µL/1x10e6 cells or 100μL of whole blood

Comment:

Anti-CD19 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

References for anti-CD19 antibody (FITC) (ABIN6655429)

Bradbury, Goldmacher, Tedder: "The CD19 signal transduction complex of B lymphocytes. Deletion of the CD19 cytoplasmic domain alters signal transduction but not complex formation with TAPA-1 and Leu 13." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 6, pp. 2915-27, (1993) (PubMed).

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background:

Synonyms: B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19

Background: CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Gene Name: CD19

Gene ID: 930

NCBI Accession: NP_001171569

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway